PCR fluorescence: the documented application
Real-time fluorescence quantitative PCR instruments are a documented application for fluorescence filters. In such an instrument, fluorescence is read repeatedly during amplification, and the filter set separates excitation light from emission light so that weak emission can be measured against strong excitation. The documented application fields for this instrument application are molecular biology, food safety testing, and public health epidemic monitoring. Those fields describe where the instrument application is documented. They are not a list of customers, instruments, or installations, and no customer name or instrument brand is published on this page.
How the filter fits the instrument
A PCR fluorescence filter set is built from pieces that work together: an excitation element that passes the light used to stimulate the sample, an emission element that passes the light the sample gives back, and related elements that keep unwanted light out of the detector path. The documented fluorescence filter evidence covers this assembly: IAD hard coating, five excitation and emission examples, blocking figures, a slope figure, dye and probe names, and sizes. The full evidence table sits on the fluorescence filters page, and it belongs to fluorescence filters only. This application page does not repeat that table as an application guarantee, because an application description and a product specification are different things: the application tells you where the filter is used, and the specification tells you what the filter does.
Selecting for a PCR application
Selection for a PCR application starts with the dye or probe your assay uses. Check that name against the documented list on the fluorescence filters page: FAM, SYBR Green, Green, HEX, TET, Cy3, JOE, ROX, Cy3.5, Texas Red, Cy5, LC Red640, and Cy5.5, with others in the same documented list. Then check the excitation and emission example that matches it, then confirm size and whether you need single pieces or a cemented filter set. The fluorescence filter selection guide walks through that sequence, and the dye selection note explains selection by dye and probe name in more detail.
What to confirm in your inquiry
Please confirm the following items in your inquiry before ordering: the dye or probe name your assay uses; the excitation and emission requirement for your instrument; the blocking range you need; the size you need; whether you need single pieces or a cemented filter set; and the quantity basis and delivery basis for your order. Please confirm the specification, quantity basis, and delivery basis in your inquiry before ordering. For the product evidence, see fluorescence filters.